PMC

Evaluation for ethyl caproate productivity of tetraploid strains with different number of the FAS2‐G1250S mutation.
A. Growth curve of each strain. The OD₆₀₀ value was monitored during cultivation in YPD10 medium without cerulenin.
B. Productivity of ethyl caproate. At the end of cultivation, concentrations of ethyl caproate in supernatants were measured using GC/MS and productivities were calculated by dividing the ethyl caproate concentration with the OD₆₀₀ value. Values are presented as means ± standard deviations from two independent experiments.
C. Schematic outline of the trade‐off relationship between ethyl caproate productivity and cell growth rate. The FAS2‐G1250S mutation has given yeast cells high productivity of ethyl caproate at the partial expense of carbon chain elongation activity during fatty acid synthesis.

Size of lipid vesicles made from membranes containing ethyl caproate (EC) at different concentrations: (A) 0% to 50%, (B) 0.01% to 10%.

Flavour‐active esters produced by Saccharomyces yeast in wine, beer and sake. Ethyl caproate = ethyl hexanoate, ethyl caprylate = ethyl octanoate.

(a) The contents of ethyl butyrate, butyric acid and the summation of ethyl butyrate, butyric acid in seven liquors of different aging years. (b) The corresponding k values (shown in the insert) measured for the liquor samples. (c) The contents of ethyl caproate, caproic acid and the summation of ethyl caproate, caproic acid in seven liquors of different aging years. (d) The corresponding k values (shown in the insert) measured for the liquor samples.